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Gérald Thouand, Robert Marks's Bioluminescence: Fundamentals and Applications in PDF

By Gérald Thouand, Robert Marks

ISBN-10: 3319274058

ISBN-13: 9783319274058

ISBN-10: 3319274074

ISBN-13: 9783319274072

This e-book assessment sequence offers present traits in glossy biotechnology. the purpose is to hide all elements of this interdisciplinary know-how the place wisdom, equipment and services are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and laptop technological know-how. Volumes are equipped topically and supply a accomplished dialogue of advancements within the respective box during the last 3-5 years. The sequence additionally discusses new discoveries and functions. specific volumes are devoted to chosen issues which concentrate on new biotechnological items and new procedures for his or her synthesis and purification. quite often, designated volumes are edited through famous visitor editors. The sequence editor and writer will despite the fact that continuously be happy to obtain feedback and supplementary details. Manuscripts are authorised in English.

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Extra info for Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 3

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PLoS One 5(8):e12441 Measurement of Bacterial Bioluminescence Intensity … 45 94. Nguyen VH, Kim H-S, Ha J-M, Hong Y, Choy HE, Min J-J (2010) Genetically engineered Salmonella typhimurium as an imageable therapeutic probe for cancer. Cancer Res 70(1):18–23 95. Cronin M, Akin AR, Collins SA, Meganck J, Kim J-B, Baban CK, Joyce SA, van Dam GM, Zhang N, van Sinderen D (2012) High resolution in vivo bioluminescent imaging for the study of bacterial tumour targeting. PLoS One 7(1):e30940 96. Foucault M-L, Thomas L, Goussard S, Branchini B, Grillot-Courvalin C (2010) In vivo bioluminescence imaging for the study of intestinal colonization by Escherichia coli in mice.

1 Photomultiplier Tube for Bacterial Bioluminescent Detection One typical example of a vacuum phototube is the PMT tube, which is an extremely sensitive detector for light in a wide spectrum, ranging from ultraviolet to nearinfrared wavelengths. Bacterial bioluminescence, enabled by the lux reporter, normally appears as a blue-green light with a center wavelength at 490 nm; thus, it can be easily detected by the PMT tube. For instance, optical fibers have been developed based on bioluminescent bioreporters for online water pollutant detection and air toxicity monitoring [34–37].

2b) are found at the re-face side of the isoalloxazine ring. The rare cis-peptide in the luciferase active site is very distinctive and has been proven to be important for the luciferase reaction [24, 45, 47]. The hydrophobic residues Trp194, Phe6, and Ser227 that form a hydrophobic surface 50 R. Tinikul and P. Chaiyen Fig. 2 a Structure of bacterial luciferase with FMN bound (pdb: 3FGC). The mobile loop segment is indicated in dark blue. b Residues around the FMN binding site. Phe6 and Glu175 exist in two conformations are found at the si-face side of the isoalloxazine moiety near the dimethyl benzenoid ring.

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Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 3 by Gérald Thouand, Robert Marks

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