By Cooper C., Packer N.
Proteome structures Ltd, North Ryde, Australia. Describes quite a few amino acid research strategies and the way every one approach can be utilized to respond to particular biologic questions.
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Proteome platforms Ltd, North Ryde, Australia. Describes numerous amino acid research innovations and the way each one procedure can be utilized to respond to particular biologic questions.
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Extra info for Amino Acid Analysis Protocols (Methods in Molecular Biology Vol 159)
Totowa, NJ 49 50 Kochhar et al. Fig. 1. Marfey’s reagent (I) and L,L-diastereomer derivative from L-amino acid and the reagent (II). precolumn derivatization with Marfey’s reagent has found applications in many diverse areas of biochemical research (3–14) including determination of substrates and products in enzymic reactions of amino acids (see Note 1). 2. 1. Vapor-Phase Protein Hydrolysis 1. Pyrex glass vials (25–50 × 5 mm) from Corning. 2. Screw-cap glass vials. 3. 6 N HCl from Pierce. 2. Derivatization Reaction 1.
1994) Comparison of amino acid analyses by phenylisothiocyanate and 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate precolumn derivatization. Anal. Biochem. 222, 19–28. 9. Strydom, D. J. and Cohen, S. A. (1993) Sensitive analysis of cystine/cysteine using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate derivatives, in Techniques in Protein Chemistry IV (Angeletti, R. H. ), Academic, San Diego, CA, pp. 299– 306. 10. Cohen, S. A. and De Antonis, K. M. (1994) Applications of amino acid analysis derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate: Analysis of feed grains, intravenous solutions and glycoproteins.
Among the more widely employed derivatization reagents are orthophthalaldehyde (OPA) (1) and Edman’s reagent, phenylisothiocyanate (PITC) (2–5), for which there are numerous publications illustrating their utility. OPA, however, has the significant drawback in that it does not react with secondary amino acids, and some of the derivatives, notably those with Gly and Lys, are unstable. PITC, although reactive with the secondary amino acids, also produces somewhat unstable derivatives, notably with Asp and Glu, which slowly cyclize from the desired phenylthiocarbamyl moieties to their respective phenylthiohydantion derivatives.
Amino Acid Analysis Protocols (Methods in Molecular Biology Vol 159) by Cooper C., Packer N.